Intracytoplasmic sperm injection (ICSI) was known as effective method in treatments of couples who unable to be helped by conventional in vitro fertilization. In 78 treatment cycles of 78 infertile couples using ICSI performed at our infertility
clinic
between May and August 1994 were analyzed. These patients were classified two groups, andrological factor (AF) and non-andrological factor (non-AF) group. The AF group, which had abnormal sperm physiology, included oligozoospermia,
asthenozoospermia,
oligoasthenoteratozoospermia (OATS) and microsurgical epididymal sperm aspiration (MESA) patients. The non-AF group, which had abnormal oocyte physiology, included abnormal zona pellucida, poor quality of oocyte and immune factor infertile
patients. A
single spermatozoon was injected into the ooplasm of 776 metaphase Ⅱ oocytes. The fertilization rate was 44.6% (346/776) and 319 embryos were transferred. After 3 embryos transfers (93.6% of treatment cycles) 23 pregnancies were established,
i.e.
pregnancy rate of 29.4% per started cycle and 31.5% per embryo transfer. Fertilization rate of AF and non-AF group was 46.2% and 35.8%, pregnancy rate was 34.5% (20/58) and 20.0% (3/15), respectively. In order to increase the pregnancy rate,
assisted
hatching (AHA) has done after ICSI in 47 treatment cycles. Pregnancy rate of ICSI with AHA and without AHA group was 34.0% (16/47) and 26.9% (7/26), respectively. ICSI was more effective in andrological factor infertility an the pregnancy rate
was
increased by ICSI with AHA procedure.
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